D13S317: 12

D16S539: 11

D5S818: 11, 13

D7S820: 9

THO1: 8, 9

TPOX: 10

vWA: 16, 17

Amelogenin: XY

This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der（16）t（1;16）（q21;q23）, der（22）t（4;22）（q12;q13）, der（12）t（11;12）（q23;p12）, del（17）（p11）, der（19）t（5;19）（?q21;q13） and others.

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 2

PGM3, 1

The cells grow in aggregates, thus cell counts are not accurate.

The cells stain positively for cytokeratins.

The line can be adapted to grow in shaker flask or spinner flask systems.

The N-myc gene is amplified, and there is expression of the mRNA and protein.

C-myc mRNA, but not protein, is expressed at a low level.

There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.

recommended serum:ATCC 30-2020

derivative:ATCC CRL-11351

purified DNA:ATCC HTB-119D

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HTB-119 NCI-H69 [H69]人肺小細胞肺癌細胞